Performing Agarose Gel Electrophoresis - Edvotek Video Tutorial

Edvotek Instructional Video: Performing Electrophoresis In this video, we are going to perform electrophoresis in five easy steps. Watch our video on preparing the agarose gel tray before starting this experiment. STEP 1: PLACE the gel tray into the electrophoresis chamber. Completely SUBMERGE the gel with diluted electrophoresis buffer. Refer to Table B for recommended volumes. STEP 2: LOAD 35 to 38 µL of the dye samples into wells in consecutive order starting with the first well. STEP 3: PLACE safety cover and CHECK that the gel is properly oriented. Remember, the dye samples will migrate towards the positive electrode! STEP 4: CONNECT leads to the power source and PERFORM electrophoresis. Refer to Table C for time and voltage guidelines. Here’s a time lapse of our experiment samples migrating. STEP 5: After electrophoresis is complete, REMOVE the gel and the casting tray from the electrophoresis chamber and VISUALIZE the results. No staining is necessary. And here you see the results!

Channel: Edvotek Inc.

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Keywords: electrophoresis, science, DNA electrophoresis, DNA, dye, dye electrophoresis, EDVOTEK, agarose gel, agarose gel electrophoresis, gel electrophoresis, agarose, separation, STEM, technology, engineering, mathematics, Math, education, science education, STEM education, DNA fingerprinting, fingerprinting, genetics, demonstration, time lapse

Id: lgmq_HsuZIU

Length: 2min 32sec (152 seconds)

Published: Thu May 30 2013