Preparing an Agarose Gel For Electrophoresis - Edvotek Video Tutorial

Edvotek Instructional Video: Preparing the Agarose Gel Tray In this video, you are going to prepare the agarose gel tray necessary for electrophoresis in seven easy steps. For electrophoresis, you will need: • A horizontal electrophoresis apparatus • A DC power source • A micropipette or transfer pipet • Agarose powder • Electrophoresis buffer • And samples for the experiment Let’s get started! STEP 1: DILUTE your concentrated buffer with distilled or deionized water. Refer to Table A for the specific amount according to your casting tray’s size. STEP 2: MIX agarose powder with your diluted buffer solution in a 250 ml flask. STEP 3: DISSOLVE the agarose powder by boiling the solution. MICROWAVE the solution on high for one minute. Carefully REMOVE the flask from the microwave and MIX by swirling. Continue to HEAT the solution in 15-second bursts until the agarose is completely dissolved. The solution should look clear like water. STEP 4: COOL agarose to 60° C with careful swirling to promote the even dissipation of heat. STEP 5: While the agarose is cooling, SEAL the ends of the gel-casting tray with the rubber end caps. PLACE the well template into the appropriate notch. STEP 6: POUR cooled agarose solution the prepared gel-casting tray. The gel should solidify within 20 minutes. The gel will stiffen and become less transparent as it solidifies. STEP 7: REMOVE the end caps and comb. Take particular care when removing the comb and end caps to prevent damage to the gel. And now you have your gel prepared and ready for electrophoresis! Now continue to “Performing Electrophoresis”.

Channel: Edvotek Inc.

Views: 128,093

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Keywords: electrophoresis, science, biotechnology, Gel Electrophoresis, STEM, technology, engineering, Math, agarose gel, agarose gel electrophoresis, EDVOTEK, DNA, DNA separation, agarose, science education, STEM education, education, dye, dye separation

Id: EZjNuqSEPbY

Length: 3min 4sec (184 seconds)

Published: Thu May 30 2013