RL: All eukaryotic cells, from yeast to those that make up the human body, contain membrane-bound organelles with specialized functions. Mitochondria are double-membraned organelles that harness most of the energy that cells need to grow and reproduce. Nearly all of this energy comes from reactions that take place at the inner mitochondrial membrane. One of the key roles of this membrane is to act as a barrier to positively charged particles called protons, thus allowing a concentration gradient to be maintained where the intermembrane space has far more protons than the matrix. The membrane also contains a large protein complex called the F1F0 ATP synthase, which uses the proton gradient to drive the synthesis of ATP molecules. These ATP molecules ultimately provide the energy for most of the cell's reactions. Just as man-made power plants produce electrical energy by using the flow of wind, water, or steam to rotate a turbine, the synthase makes ATP by using proton flow from one side of the inner membrane to the other to rotate protein subunits. If there is no proton gradient, synthase subunits stop rotating, and the cell can quickly become starved of the energy and die. Therefore, the protein complexes and small molecules that establish this gradient and maintain it play an essential role in the life of the cell. At the heart of this system are four protein complexes numbered I through IV. Complexes I, III, and IV directly pump protons from the matrix into the intermembrane space. Complex II does not directly pump protons, but it does promote proton pumping in complexes III and IV. Proton pumping requires energy, and the four protein complexes get this energy by transferring electrons through a series of coupled reactions. This linked process of electron transport is why the four complexes are collectively referred to as the electron transport chain. Let's focus on complex I. A byproduct of sugar metabolism called NADH deposits two high-energy electrons in complex I, where they are passed along a chain of redox centers. Redox centers are clusters of atoms that have different affinities for electrons based on their unique atomic configurations. Let's closely consider a pair of redox centers to reveal two reasons why an electron moves from the top redox center to the bottom. First, the bottom redox center has higher affinity than the top one. Second, the distance between these adjacent redox centers is ideal for an electron jump to occur, which explains why electrons typically don't bypass the bottom redox center. A small amount of energy is released each time an electron is passed between redox centers. Complex I harnesses this energy across all the redox centers and uses it to pump protons. The last redox center in complex I donates two electrons to a coenzyme Q molecule. Complex II is similar to complex I in two important ways. First, high-energy electrons also enter complex II via a byproduct of sugar metabolism, although here the molecule is FADH2. Second, complex II also transfers electrons between several redox centers before donating them to coenzyme Q. One major difference, however, is that complex II does not use the energy liberated to pump protons. Coenzyme Q molecules from complexes I and II donate their electrons to complex III. One electron is a recyclable and can re-enter complex III later, but the other passes through two redox centers before reaching cytochrome c. Cytochrome c carries the electron to complex IV. The electron transport chain ends in complex IV, where a series of reactions involving four electrons converts a molecule of oxygen to two molecules of water. The proton gradient is strengthened because four protons from the matrix are incorporated into water molecules, and another four are pumped into the intermembrane space. In the absence of oxygen, the electron transfer comes to a halt, meaning that ATP synthesis also stops. Indeed, the reason we breathe oxygen is so that it can serve as the final electron acceptor at the end of the electron transport chain. In this animation, we have explored each protein complex in isolation, but in reality, they are very densely packed. Together, they effectively make the entire surface of the inner mitochondrial membrane a giant cellular power plant.
This is oddly the secret to multi-cellular life, and a fascinating piece of the mystery to evolution. This is the process by which the mitochondria -- the powerhouse of the cell -- generates the large amounts of ATP for our cells, and has allowed eukaryotic life to push beyond the bounds of more simple lifeforms.
I do enjoy these protein simulation videos from the HarvardX channel, as they really capture the bizarre mechanical nature of cellular life, something that you can't really appreciate on the scales that we normally operate on.
Love the ETC. LOVE explaining it to high schoolers, especially during the aerobic unit. After they had an understanding, I’d show this:
https://m.youtube.com/watch?v=VCpNk92uswY
It’s so GOOD!
As a layman with a passion for evolution, this video totally blows my mind. The fact that anything works and is alive is literal magic.
Very cool video. I had never heard of the ETC or realized it's importance for evolutionary biology until reading this book that came out last year: https://global.oup.com/academic/product/mitonuclear-ecology-9780198818250?cc=us&lang=en&
It's an academic publisher but pretty down-to-earth comprehensible style of writing.