RL: All eukaryotic cells, from yeast to those that make up the human body, contain membrane-bound organelles with specialized functions. Mitochondria are double-membraned organelles that harness most of the energy that cells need to grow and reproduce. Nearly all of this energy comes from reactions that take place at the inner mitochondrial membrane. One of the key roles of this membrane is to act as a barrier to positively charged particles called protons, thus allowing a concentration gradient to be maintained where the intermembrane space has far more protons than the matrix. The membrane also contains a large protein complex called the F1F0 ATP synthase, which uses the proton gradient to drive the synthesis of ATP molecules. These ATP molecules ultimately provide the energy for most of the cell`s reactions. Just as man-made power plants produce electrical energy by using the flow of wind, water, or steam to rotate a turbine, the synthase makes ATP by using proton flow from one side of the inner membrane to the other to rotate protein subunits. If there is no proton gradient, synthase subunits stop rotating, and the cell can quickly become starved of the energy and die. Therefore, the protein complexes and small molecules that establish this gradient and maintain it play an essential role in the life of the cell. At the heart of this system are four protein complexes numbered I through IV. Complexes I, III, and IV directly pump protons from the matrix into the intermembrane space. Complex II does not directly pump protons, but it does promote proton pumping in complexes III and IV. Proton pumping requires energy, and the four protein complexes get this energy by transferring electrons through a series of coupled reactions. This linked process of electron transport is why the four complexes are collectively referred to as the electron transport chain. Let`s focus on complex I. A byproduct of sugar metabolism called NADH deposits two high-energy electrons in complex I, where they are passed along a chain of redox centers. Redox centers are clusters of atoms that have different affinities for electrons based on their unique atomic configurations. Let`s closely consider a pair of redox centers to reveal two reasons why an electron moves from the top redox center to the bottom. First, the bottom redox center has higher affinity than the top one. Second, the distance between these adjacent redox centers is ideal for an electron jump to occur, which explains why electrons typically don`t bypass the bottom redox center. A small amount of energy is released each time an electron is passed between redox centers. Complex I harnesses this energy across all the redox centers and uses it to pump protons. !!!___For Clarity___!!!
The membrane arm of complex I is omitted. only the peripheral arm is shown in the animation The last redox center in complex I donates two electrons to a coenzyme Q molecule. Complex II is similar to complex I in two important ways. First, high-energy electrons also enter complex II via a byproduct of sugar metabolism, although here the molecule is FADH2. !!!___for Clarity___!!!
FADH2 does not leave complex II but it`s a prosthetic group that binds tightly to the protein. Succinate is the electron donor to FADH2. Second, complex II also transfers electrons between several redox centers before donating them to coenzyme Q. One major difference, however, is that complex II does not use the energy liberated to pump protons. Coenzyme Q molecules from complexes I and II donate their electrons to complex III. One electron is a recyclable and can re-enter complex III later, but the other passes through two redox centers before reaching cytochrome c. Cytochrome c carries the electron to complex IV. The electron transport chain ends in complex IV, where a series of reactions involving four electrons converts a molecule of oxygen to two molecules of water. The proton gradient is strengthened because four protons from the matrix are incorporated into water molecules, and another four are pumped into the intermembrane space. In the absence of oxygen, the electron transfer comes to a halt, meaning that ATP synthesis also stops. Indeed, the reason we breathe oxygen is so that it can serve as the final electron acceptor at the end of the electron transport chain. In this animation, we have explored each protein complex in isolation, but in reality, they are very densely packed. Together, they effectively make the entire surface of the inner mitochondrial membrane a giant cellular power plant. Thanks for continuing to the end.
You can watch the rest of the videos where you`ll find the other information about mitochondria and most of their Reactions.
The powerhouse of the cell
So awesome. This was discussed heavily in a fantastic recent podcast:Peter Attia MD & Vamsi Mootha, M.D.: Aging, type 2 diabetes, cancer, Alzheimer’s disease, and Parkinson’s disease – do all roads lead to mitochondria?
The beauty of life itself is humbling. A stark contrast to the behavior of humanity in 2019. How does something this beautiful get so ugly ?
Watch turning proteins create stored chemical energy (ATP) from electrons !
Mitochondria are 2 billion years old.
https://youtu.be/lhF5G2k45vY
and are the basis of life beyond single cell organisms.
Here's the original (1080p) video https://youtu.be/LQmTKxI4Wn4
Cool video, but I think it's worth noting that NADH and FADH2 are not "byproducts of sugar metabolism".
Fatty acid beta-oxidation also converts NAD+ into NADH and FAD into FADH2.
Also ketone metabolism feeds into the citric acid cycle, so no glucose required.
Absolutely magical
Fun fact: Endosymbiosis is the theory that mitochondria were bacteria which evolved a symbiotic relationship with eukaryotic cells, and that theory was proposed by Dr. Lynn Margulis, who was also a 9/11 Truther.
Excellent, I think I actually understood that! Great illustrations