What Is Capillary Electrophoresis?

[Music] capillary electrophoresis is a method for separating and identifying fragments of fluorescently labeled DNA by passing them through very thin polymer filled capillaries the use of capillaries instead of a conventional slab gel makes it possible to apply a much stronger electric field resulting in faster separation and higher throughput the process features single based resolution and requires small sample quantities applications include STR analysis and Sanger sequencing as well as many other everyday applications used by life science and forensic laboratories here's how it works first fluorescently labeled DNA samples are prepared for electrophoresis and loaded into the seedy instrument a capillary array is filled a column ER and placed into the sample wells when the capillary array enters the wells an electric current is formed and the DNA fragments are moved electric kinetically into the capillaries next the ends of the capillary array are inserted into the cathode and anode buffer the instrument applies a specific voltage and temperature to control sample migration through the capillary array the fragments are separated by size with the smallest fragments moving and fastest the final step is sample detection as a fluorescently labeled fragments move past the detection window the laser excites the fluorescent dyes the emitted light is spectrally separated and recorded by a CCD camera the CCD camera records the fluorescence signature of each fragment as well as the elapsed time for the fragment to migrate through the polymer the raw data is sent to the collection software in the form of a peak and is now ready for analysis using your choice of software program this animation is brought to you by spectrum compact seee system a small but mighty seee instrument for your lab [Music]

Channel: Promega Corporation

Views: 54,780

Rating: 4.8993134 out of 5

Keywords: dna, sequencing, capillary electrophoresis, str analysis, ce, genetic identity, capillary, electrical, electrophoresis, separation

Id: x7PUqNA0eOA

Length: 2min 15sec (135 seconds)

Published: Wed Aug 01 2018