Crispr cas9 gene editing explained

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\paperw12240\paperh15840\margl1440\margr1440\margt1440\margb1440\gutter0\ltrsect \widowctrl\ftnbj\aenddoc\trackmoves0\trackformatting1\donotembedsysfont0\relyonvml0\donotembedlingdata1\grfdocevents0\validatexml0\showplaceholdtext0\ignoremixedcontent0\saveinvalidxml0\showxmlerrors0\horzdoc\dghspace120\dgvspace120\dghorigin1701 \dgvorigin1984\dghshow0\dgvshow3\jcompress\viewkind1\viewscale100\rsidroot11419918 \nouicompat \fet0{\*\wgrffmtfilter 2450}\nofeaturethrottle1\ilfomacatclnup0{\*\docvar {dgnword-docGUID}{\'7b4A8938D3-1D88-41C0-9B5D-1568CD955E76\'7d}} {\*\docvar {dgnword-eventsink}{1631435951616}}\ltrpar \sectd \ltrsect\linex0\sectdefaultcl\sftnbj {\*\pnseclvl1\pnucrm\pnstart1\pnindent720\pnhang {\pntxta .}}{\*\pnseclvl2\pnucltr\pnstart1\pnindent720\pnhang {\pntxta .}}{\*\pnseclvl3 \pndec\pnstart1\pnindent720\pnhang {\pntxta .}}{\*\pnseclvl4\pnlcltr\pnstart1\pnindent720\pnhang {\pntxta )}}{\*\pnseclvl5\pndec\pnstart1\pnindent720\pnhang {\pntxtb (}{\pntxta 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If you see last is a research \hich\af1\dbch\af31505\loch\f1 is crisp}{\rtlch\fcs1 \af1\afs24 \ltrch\fcs0 \f1\fs24\cf1\insrsid11419918 \hich\af1\dbch\af31505\loch\f1 r cas9 }{\rtlch\fcs1 \af1\afs24 \ltrch\fcs0 \f1\fs24\cf1\insrsid2972498 \hich\af1\dbch\af31505\loch\f1 reality is crisp system is that she system zero zero EDT system insisted system which can destroy DNA, which is present inside the said eukaryotic cell mostly also's makes more sense. So that means this sign can catch a target DNA specific locati \hich\af1\dbch\af31505\loch\f1 on specific DNA in specific location and the dealer we can DNA totally destroyed his is also the try to fix that the physician of error}{\rtlch\fcs1 \af1\afs24 \ltrch\fcs0 \f1\fs24\cf1\insrsid11419918 \hich\af1\dbch\af31505\loch\f1 }{\rtlch\fcs1 \af1\afs24 \ltrch\fcs0 \f1\fs24\cf1\insrsid2972498 \hich\af1\dbch\af31505\loch\f1 prone process may introduce some sort of mutations of nucleotide into the geo-and patient and changing organism we can mo \hich\af1\dbch\af31505\loch\f1 dify it in many different ways. So is this the second is gas station. I. The idea is this's physical structure small segment of the structure. I is routine in. He is a sequences of the TSA's template and contains a demonstration of what is this because I \hich\af1\dbch\af31505\loch\f1 i\hich\af1\dbch\af31505\loch\f1 s normally in this particular video explaining the step-by-step manner different, so talk about the history and office for National talk about of an talk about the Hughes) \par \hich\af1\dbch\af31505\loch\f1 future times origin of this percussion insist you that this system is a basic has en\hich\af1\dbch\af31505\loch\f1 \hich\f1 ormous capabilities of how this crisp issue that at all, and we want the top of the top of the right topics brings this is a\'e7\loch\f1 ai this crisp is the I is the top nine can keep accepts cash strike stranded in a six will only pick the. The crisp is the temple.,\hich\af1\dbch\af31505\loch\f1 Which is provided and need to match the precipice with the target to clean right target DNA and specifically sequence which is complementary to this recently this because of the complementary structure of finally I inactivated is always associated with t \hich\af1\dbch\af31505\loch\f1 h\hich\af1\dbch\af31505\loch\f1 is nine has simply this is standing up for the stand at a particular location and get a target DNA completely broken down from the Centre contemplated is far from the centre point, so this will is lost and damaged our fix it in this double-stranded play t \hich\af1\dbch\af31505\loch\f1 h\hich\af1\dbch\af31505\loch\f1 en be used to methods of the double-stranded break are now enjoying the best accomplished this process of a combination of enjoying particularly the normal century. This is probe that the process may this position may be lost of each and which may contain \hich\af1\dbch\af31505\loch\f1 \hich\af1\dbch\af31505\loch\f1 some of the more wanted something totally different, so this modification, the most chains transcriptions of cheese and this is also altered the hands of the producing strong portion of protein previously bit of truncated version of the protein function a \hich\af1\dbch\af31505\loch\f1 l\hich\af1\dbch\af31505\loch\f1 so the origin of the small copy of the protein is produced in in respect of the large amount of putting the people to the supposed this is what is happening into this crisp assessment system I moved so what we need to pass this thing is we can design this \hich\af1\dbch\af31505\loch\f1 \hich\af1\dbch\af31505\loch\f1 in such a way that we have structure of an crisp and complex will be scanned for that structure. The target DNA. Will keep the team in an open sports right because we typically destroy daily, so we can some wheel, so the total DNA content can be changed. \hich\af1\dbch\af31505\loch\f1 A\hich\af1\dbch\af31505\loch\f1 s you can paralysed with this system with some sort of recent act specifically is a specific period of geo-complicated organism like us get so busy I and when this is because I idea was to very early in his bottleneck from the 1990s get a much better that \hich\af1\dbch\af31505\loch\f1 \hich\af1\dbch\af31505\loch\f1 he is focusing on genetic is this a sign system depends on of the bacterial bacterial ability of human rights support directly, which also has its own yield by farms points) of protect themselves from attack gas nine crisp and was all inside bacteria. We \hich\af1\dbch\af31505\loch\f1 a\hich\af1\dbch\af31505\loch\f1 re trying to replicate the same process to give interested in is the bacterial cell, and is this is transferring its general secretary contents of less than his impeccable proposals, so this is fast DNS. There are some places where we have this sequence. \hich\af1\dbch\af31505\loch\f1 T\hich\af1\dbch\af31505\loch\f1 his sequence sequence of this crisp type of our normally this contest for. Can you simply are ready to be like in contact with some responsible structures from outside the complement of the past DNA and refuse with the region's first points of this DNS sy \hich\af1\dbch\af31505\loch\f1 s\hich\af1\dbch\af31505\loch\f1 tem as per structures is already present inside with the help of this region is the region of is somehow kill survives in the future. This bacteria is us for the next time over the same Texas and the antibodies are already ready and the same thing is to i \hich\af1\dbch\af31505\loch\f1 n\hich\af1\dbch\af31505\loch\f1 case of facts on genetic material is available inside that is already in prison copy of the crisp I see are this crisp is produced. This crisp images of this cast or more complex and from this complex complexes will interact with this responsibility real \hich\af1\dbch\af31505\loch\f1 l\hich\af1\dbch\af31505\loch\f1 y know that the final region sporting crisp is a show that is utilised to our structure structure can be. This is why complementary structure computer structure as per our system from is simply can perfectly sometimes are not perfectly represent, but stil \hich\af1\dbch\af31505\loch\f1 l\hich\af1\dbch\af31505\loch\f1 \hich\f1 action triggers that a\'e7\loch\f1 \hich\f1 ai the a\'e7\loch\f1 ai the final which is clearly spelt this is the bacterial process and in this process and advantage of this process. The theme always thought about how can the reason behind it is very clear and that same complementary fea\hich\af1\dbch\af31505\loch\f1 t \hich\af1\dbch\af31505\loch\f1 ure strand in the use of complementary to this idea is utilised for an system of units and breaking down the DNA structure so modify this crisp from outside. If we can provide complement from a outside. For example, this is like this, so if this is partic \hich\af1\dbch\af31505\loch\f1 u\hich\af1\dbch\af31505\loch\f1 larly structure for this contains the complementary structure. You see, you should find a complementary. This is a crisp settled by protein will destroy this particular DNA structure in the stack structure of the Straits Times of this is like the mechanis \hich\af1\dbch\af31505\loch\f1 m\hich\af1\dbch\af31505\loch\f1 of how exactly is focused on books. Now the mechanism of this is more economical in cases that the deal is, and this target DNA structures, particularly crisp video to see our crisp is sometimes used alone, gas nine yes nine sometime this is also, in whi \hich\af1\dbch\af31505\loch\f1 c\hich\af1\dbch\af31505\loch\f1 h he see as per gas situation is actually when we talk about this system is important of research information is only every single day. That's why you S9 is the SI system also transfer transitivity conceptual box, this that you want to discuss regional. \par \hich\af1\dbch\af31505\loch\f1 We\hich\af1\dbch\af31505\loch\f1 focus on the completely and specific catalytic site and special G sequences of the this and keep cities for the specific region GG. This site is an particular structure in GG this particular structure is a specific sequence presented side-by-side. This i \hich\af1\dbch\af31505\loch\f1 s\hich\af1\dbch\af31505\loch\f1 the place. This military key processor and stretch of the witch is I is Spitzer is in the process a little region the process down the stretch of the DNA CR nine and long complementary structure to Spitzer is known as only space and the law. This is stru \hich\af1\dbch\af31505\loch\f1 c\hich\af1\dbch\af31505\loch\f1 ture so structure. \par \hich\af1\dbch\af31505\loch\f1 This is because I wish & get access in Moti he the strength of the so there are different versions and types of in mechanism and personal this stage of this here is known as only the interest is trans-activating crisp and structure like \hich\af1\dbch\af31505\loch\f1 that and his focus system only is connecting the structure of the crisp and was find the complement the region. Then I see weekly weekly, three of which company for this kind of structure actually not symbolising the state is the this particular structure .\hich\af1\dbch\af31505\loch\f1 This is because I'm pretty necklaces are sites I hear here. This specific niche of this job and like in the absence DNA structure yield a S9 sometimes also the now important point about the use of breakage. So in simple terms you like what is importance \hich\af1\dbch\af31505\loch\f1 o\hich\af1\dbch\af31505\loch\f1 f this because I this point, but I can say in your second destroy the target DNA. If you want to destroy the company silencing of the sea use this specific see that we design in vitro and in Cyprus and they can keep the sign that is above the gene silenci \hich\af1\dbch\af31505\loch\f1 n\hich\af1\dbch\af31505\loch\f1 g. This is one of jobs silencing is one of the importance of system was discovered. The first is that it can be fixed with a combination of the century complicated and so in between, which is not supposed to present its reading frame will be changed to th \hich\af1\dbch\af31505\loch\f1 e\hich\af1\dbch\af31505\loch\f1 production of the protein will be changed. The protein properly functional truncated protein may not be functional limited capability which we can change the transcript inside the said we can change how exactly the professor produces at this, so this sor \hich\af1\dbch\af31505\loch\f1 t\hich\af1\dbch\af31505\loch\f1 of can be done own analysis and modification and modification can be silencing the are also associated here about the destruction of this complex is simple in Cyprus completion of the units on the weekend. Scientists believe that this crisp has more pote \hich\af1\dbch\af31505\loch\f1 n\hich\af1\dbch\af31505\loch\f1 tial and its shutdown activity to shut simply crisp complex associate is currently it will not additional factors from outside transcriptional eukaryotic transcription, translation transcriptional activator molecules can initiate transcription of the spec \hich\af1\dbch\af31505\loch\f1 i\hich\af1\dbch\af31505\loch\f1 fic gene products that you wish, so we I we can transcribe DNA region percent and the RNA from that stage of the yes we can so important use is we can allow selective transcription selective transcription is similarly also components transcription of spec \hich\af1\dbch\af31505\loch\f1 i\hich\af1\dbch\af31505\loch\f1 fic region of the system. If we inside his own, which will be required on the east of, the will not be liable for the transcription start the transcription. We can also utilise this is my structure accessory protein to limit the transcription in this case \hich\af1\dbch\af31505\loch\f1 \hich\af1\dbch\af31505\loch\f1 is transcription division edition of transcription in this specific provision means stretch of the complete general section of genes right. This was not formally gas nine so many things right so that is why as a Pandora's box important biological research .\hich\af1\dbch\af31505\loch\f1 Since that can be done simply this hybridisation process. I can interact with the PLA and hybridisation system. This site of the regions of the S9 of protein in a way she whispered into the 20 percent to the protein location specific genes, so we can lit \hich\af1\dbch\af31505\loch\f1 e\hich\af1\dbch\af31505\loch\f1 rally campaign Jimbo can be done and also if you want to find the regional location of specific regions in the week and of different types and different of frozen With the help of this crisp perspectives. So opportunities limitless break one say is that a \hich\af1\dbch\af31505\loch\f1 \hich\af1\dbch\af31505\loch\f1 particular gene is not working properly working properly disease. For we can design complementary target crisper RNA is because the insistent that particular gene and the strategy strategy that disease can be reversed different problems associated's this \hich\af1\dbch\af31505\loch\f1 k\hich\af1\dbch\af31505\loch\f1 ind of thing is we can also incorporate nucleotides in specific locations. We can introduce mutations in the cases gene therapy right. The importance of nine so six crikey is useful for gene therapy can also utilise. 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Channel: Shomu's Biology

Views: 56,246

Keywords: suman bhattacharjee, shomus biology, gene editing, genome editing, crispr cas9 protocol, genome engineering, crispr cas9, crispr cas9 explained, crispr cas9 gene editing, crispr cas9 experiment, crispr cas9 system, crispr cas9 knockout, crispr cas9 mechanism, crispr, cas9, crispr-cas9, crispr genome editing, crispr explained, genetic engineering, crispr cas9 gene editing in plants, crispr cas9 gene editing explained, crispr cas9 gene editing technology

Id: OPkPwNVNLEs

Length: 31min 6sec (1866 seconds)

Published: Fri Oct 09 2020