Casting an Agarose Gel

in this video you will learn how to cast an agarose gel start by taping both ends of a gel tray with laboratory or painters tape making sure to securely seal the edges of the tray press down firmly along each edge of the tape to ensure that there are no leaks repeat this procedure with the other side of the gel tray again making certain that each edge has been firmly sealed next place the comb into the comb guides on the gel tray if you plan to run DNA samples the comb should be placed at one end of the gel tray place the tray with comb on a flat and level surface before pouring the molten agarose make certain that it has cooled to 55 degrees Celsius pour the agarose into the gel tray until the agarose comes to within two to four millimeters of the top of the teeth of the comb when the gel is cool and opaque remove the comb by pulling it gently straight up and out of the wells you should be able to see that each well is well formed if the comb is removed before the jello set the wells will collapse if the gel is to be used immediately remove the tape from both ends of the tray and continue with the activity you if the gel is to be stored for later use keep the gel in the gel tray leaving the tape on and store it at 4 degrees Celsius in a small plastic bag you

Channel: Bio-Rad Laboratories

Views: 138,989

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Keywords: bio-rad, life science, education, biotechnology, biology, molecular biology, technique, method, student, teacher, school, classroom, laboratory, laboratory skills course, textbook, lab, activity, demo, demonstration, instuctional, instruction, electrophoresis, pour, set, cast, tape, tray, comb, stem, dna, ngss

Id: KKmiKKMDDhY

Length: 2min 26sec (146 seconds)

Published: Tue Oct 16 2012