Koehler Illumination - Setup & Alignment | by Motic Europe

hello my name is doctor answered Clemens from Ooty Kira and in this video I'd like to show to you how to do the proper cooler set up on our be a fortune model this explanation will be valid also for the BA 310 310 a liter and the respective a liter model from bf4 10 what's the idea of the CUDA setup the idea of the CUDA setup is located high quality illumination with a homogeneous background improved contrast and reduced stray light so how to do it first of all of course switch on the microscope the main switch is on the back of the microscope take your sample and place it onto the stage move the XY stage until the sample is in the rape of swing in the 10 times objective which is coated with a yellow color adjust the interpupillary distance individually for your eyes only and focus until the image is getting sharp please make sure that the condenser head of the bf4 10 is in the rape of the feed diaphragm is an integral part of every color setup it is built-in in the base of the microscope you have to close it until the edges of the field I frame are visible in your eyepieces now please focus the image of the field diaphragm to the point where the image is getting sharp means that the edges of the field diaphragm are as sharp as possible if the spot of light is not concentrated in the middle you have to move the diagonal screws on the condenser carrier until the spot is moved to the middle of the field of view open the field diaphragm up to the point where it's not visible at all the last step of a proper cuda setup will be the correct use of the approach' diaphragm which is built in part of the condenser closing the diaphragm will increase contrast but reducing resolution opening the diaphragm will increase resolution but reducing contrast so please use this diaphragm carefully the complete procedure of focusing adjustment of the feel diaphragm and adjustment of the condenser very well at the same time centering the illumination has to be repeated for each objective separately if you'd like to see more tutorial videos please visit our youtube channel or other social media platforms or just visit our website multi cure up calm thanks for your attention see you next time

Channel: Motic Europe

Views: 42,402

Rating: 4.9507909 out of 5

Keywords: Motic, Motic Europe, Microscope, mikroskop, BA310 upright microscope, BA310E upright microscope, BA410 upright microscope, BA410E upright microscope, Koehler, Illumination, Upright microscope, Biological microscope, Koehler setup, Koehler alignment, Koehler illumination, How to, Köhler Illumination, Kohler illumination, How to use Koehler illumination, How to use köhler illumination, Koehler Illumination setup & alignment, Motic microscope

Id: dYP0uBxE0AA

Length: 3min 39sec (219 seconds)

Published: Fri May 30 2014