Ion Exchange Chromatography | Iso Electric Point | Tamil | Mechanism | Biology | ThiNK VISION | tnk

[Music] negative ion exchange chromatography paper chromatography linearity freaking camera type then the ion exchange chromatography so pristine chromatography finally in a three other people's idea when open our apathy air can be gentle analyst simplified our video Podrick and the video or a link description their first and second to enemy upon I input in a lava pool so when I'm in the curriculum oh now I'm next in the graph even some in the medical condition a separate charge to the polar molecule sergeant non-poor are not only like he doesn't know and the man even the charge is a particle is separate bundles okay now we're gonna learn the technique on the useful run a definition for thumpin any difference on the pH of the unelected so unlike no no no became a sample on a later from some partner either their crappy whichever true naman the separate from language in a charge entropy gets in trouble isn't ending but me I want the para poner they if they open desire to the power firstly the lyrical components on the bathroom chromatography leukemia moon cumference now face in the Catholicism so we can build on a tenant path or now the charge to particular unit is charged particle s upon a charged particle if it was stationary past the phase path or another on the column matrix illustration efficiently car America you could I mean there's an especially night or go up enough fixed ion up alone on this auto-ship on it and then explain now and then I'm gonna come and they'll pay you more than came on I rolled on the plate for no and the empty plate front therefore the mechanism you can stay on the chromatography ion exchange chromatography relativism of the page and only one the cation exchange chromatography ion exchange chromatography so paired let me know if there is a kimono in the positive separate Pony known the negatives of grateful say look on the coop own yet we can't anything on n is the positive negative of a vertical symmetric so dry kappaa epromos monopolist american so upon the positive allowing the separate form or another in an animal path open our indian the perot so they are negative up on now it's kinda negative on a nothing the separate paths open under 707 or something Arbor on a matrix a either good or you can the carboxy methyl on the group of a CMO from the sumo see a negative charge after them the positive will not track for homeopathy if I think a kilogram onion exchange chromatography low path or none of them in a separate part of it is the negative charge and I'll end up another cellulose on the matrix pocket 11 this Dae diethyl amine oh yeah I've even the positive charge also passed research other than a negative or not right for no I never say you know in there any type of the pre gonna so simple I cannot avail positive a separate for not cation exchange chromatography negative a separate Pune and your next is promoter you number to my in love scanner if I return the mobile phase Condor over face 11 the buffer use belong so mobile based on them and I'm a sample on the 32 I mean I prefer open to go so easily in the diaper and I poke upon the tiny de Porres on the mobile face 11 there are the reaction of the Quran rented a particular positive buffer negative of elephant a positive buffer on the traces so other than the positive ions of the separate or no negative buffer when they acetate and phosphate even the negative and some of the separate mother - oh so open the chromatography AP pondrom - or at the power kinetic unison in the pH and charge is there any link between a separate phenomena open 10 the leap under point is an isoelectric point so nice electric point 9 print our molecule now a separate phone RNA molecule on the charged I reckon operon sorry the positives are generally negative sorrow in do not relevant the neutral charge of America for particular bigots are open point of particular bigots 11 way in then they in the charge of llama the molecule of the neutral charge 11 other the net charge of the zero Oracle and in the point amount the undefeateds around in a my isoelectric point up and on this or no so on the pH scenario come over the positive or the negative over curve and the peak edge of tomorrow move it other than the positive are modular negative all other than the isoelectric focusing upon this alumina so isoelectric point other the PAI pending or phenomena up for now in there and the molecule one they charged into molecule other and on the particular pH 11 they other than their zero I want a report next charge on the neutral Africa even the open protein come tomorrow set of protein week when they up row Mahayana pH 11 this year overall same protein you in the car Domino on a pH set of the zero are considered impeller and pH 7 normal on at the net diagram departing Yamuna in the norm on of the it's around zero or it's a pronounced the negative either they are they become eh appears come here chef in a positive out of it either which moon type of and appear upon their physical state sample path to open up proteins in a protein on the point peptide chain than you know J beeps alarm you know a seed of penicillin I mean I said when they positive or vasila ominous you know the negative article as opposed to another net charge on the morrow so pH are based upon you in the three form so no apocalypse do in the positive charge in them path often huh if the pH is lesser than the isoelectric point it no more could repeat isoelectric point Orrico if I have in the diagram la paga no matter the blue color a katana isoelectric point a nose cone however that come here is a a pronounced a in the molecule of the positive charge of the morning so normally recur than the neutral or on the charge on this positive charge of the miner if I render path upon a negatively charged if the pH is higher than the isoelectric point so Pikachu an isoelectric point a better idea and it's a open up a manner the red column along Nadia might say renowned egative Sir John the Mauryan of the molecule if we can ask to a neutral out of this room today inform of her new Salonga upon Donna Pearson immersive theater it and the Pikachu Monica lot of PA of an isoelectric point when the e-collar and they say I pronounce it a landing neutral formula because for mnemonic aluminum the charge America today on a peninsula so nice electric point of epsilon P hit Sumati and money products are jamaat eat anything up in the corner and you people running up to the bar the an exchange chromatography diamond came on our state's it attend the Nara station and have all the power first space park open air conditioning admission he no no no metallic from the conditional logic a movie of the propellant is found in a large nor bow tie I mean I am from i NX is chromatography on the Holden and I can open the paper is over cuz my conditioning up on the solo so when the conditioning line never know open now and then the stationary phase to the collapse the stationary phase love and then I'm a cellulose matrix on thought silicon if you could have one brain a sudden on a fixed day and - open other than the conditioning of the Punahou explained in a happy - annapurna in the molecule among the separate Punahou on the morning of the fifth charge Electr molecule of material on the charge Panora so from the column when they are the conveying the charge they recover neutral Erica and Amanda one before now the positive on the separate negative on the charge of the player home opener on the positive were given the curio I've known the condition in division they provided the bottom of the key pointed loading the sample so a minimum sample on the bottom or the other if you'll open the VM chromatography line on this one take on the video game so I mean Oracle and them Devin Devin one more but nobody would take up on the next chromatography or the mechanism of the start so are the pulley in interactive way up into the power so conditioning loading covering for minimum separation spacing on the torso or a neuron they say poverty capital bathroom so now I'm ready for it now the matrix something about this a little a gross of the useful more I don't know the stationary phase even I'm part of the Sigma Sigma into the metrics on the independencia bottom are even the bomb so the benefit is they wha relevant number condition program in the conditions when you go articles it charge you could go in phenomena partners on the calculate still chromatography khaki another historian that's positive is the prefrontal knowledge cation exchange chromatography about that so positive separate from no no no Google Erica molecule no the negative charge and not end up in your corner negative charge of neither of the my cute sir commander negative I am of the cm fluoridation the carboxy methyl group so other any path on a condition when your Sigma u negative solid empirical if a node for example I wrote front of both of the sample on this negative I open it cut unless I make a positive and a green color it is a neutral molecule and all along the diagonal if a lot finito Oh Monica great now going for Samsung you've given to move on so a random buffer which is Pokemon about a sentence in the number of sample within this area more Moomba the in another corner a more positive and then the negative says already act when you move me to I want to move on then when enamel or a neutral charge I put on negatives as they don't move em on the energy level I think I pronounced it in the positives are around in the negatives hazard on the in fact in Kunene siphon even they have been in the auto market a you a personal opinion but on the neutral charge of and their economic some romantic Spanish although Allah Almighty the money even thing and even the mighty Neko is even a negative other than the other whatever see come up maybe a moment the killer you know so you put it on there on the separation of another over molecule of ostrich Allah the Almighty through a negative charge of the way my initial we may an ionic exchange chromatography path of ninety learn how the opposite turn in your silver positive charge of sumo Pucci look at the number of the negative charge around zero so however separation Linotype regalia and path of count three who's penny separate from another even if I become prey and the ato spent on happen I think I'm a mu not about the technique lighten up on more we're on this editor semantics on there as a good athletes day and you spent on that other day director more Donnelly Parvati take and the problem the police say to Paul Romer Diego put a complication to home I think my path of burglary use pornography easy I'm to degrade right there fo are the world compared to exam within the conference Pournami the entire comprehendible path or not so are they my Ravana matrix on they require good then I'm going to expand the covalent bonds love that Britain can so covalent bond delightful imposes and stronger that's a good on the counter I am on the exam the counterion so no me other with a separate one from oh and the molecule of the separate from no more as a molecule charged along the counter a manwich a pocket so I found them a positive a separate from no are they positive charge long chiffon on inv in the corner corner ionic bond 11 0 I mean keep me comment there go so don't even ever go and I might with remove becoming the procedure go so it when I'm going on a little apart on wrote a sample of on another separate phenomena as a positive molecule Manaus Don bundle of that si remarks which my example consider it Dakota border again Istanbul comma so on the merry you compose a novel for not either we cavity as an economic exchange contrivance of bees even to control either with the variable solar or any purple nose in the beaker and the horse another car in there I am with the very acuerdo so are they meant number of negative of the pine tar the new combined together now have there any before a new time so paying union a colectomy exchange on their own it's a number on a little positive on there in the contrary on exchange money in everybody else's so use even the point of in the form of and you know so no move italiana positive charge and then I'm a separate one from using a on ionic exchange chromatography that is a another calm so no more requiring about the matrix of they are they practical moving of the positive which is counter the negative of the uniform so on a little ok negative Eriko now in a meeting with the tiniest separator even there however I'm not even on this plan pointed about that's I keep even the radiator ain't never gonna happen today so in the following una either would a stronger bond on your career or molecule at the a charge the molecule on that opponent if probably in your positive up in a lot stronger bond diagnostic on either better stronger bond tire or positive molecule on the corner and more the either with the current the other work you know so even have been I moved away on a variable they updated Sigma so everyone the Aleutian washing process rather than I to the power so mechanism doing the separate talk of the up front of the bathroom fight at the moon came honest dependent path or not washing so in the Washington anima path Oprah now and they removing the unbound samples so minimum Khepera material and sample animate 20 character than their stationary phase long devote a trip or if I'm both us in other when the market you want anger and the movement of the cleaner the more than the washing step on the porno so you know the table are compounded Lamia when they wash a medium three more you find it the knowledge step path of another man came one of the given animal rode on the someone say when they use Punto in the isoelectric point I love asana in upon drying up now a buffer of pH 7 the matamata Molina the empanada mangu bigots March and the charge from the underneath of the charge March automatic say hakuna other the interaction of tomorrow soapy get some a stronger and the isoelectric focusing in our community when they separate monomania Malik rota isoelectric point when the number of this video so an isoelectric point about at pH 7 the idea proton I love the company owner if I work together on the positive are okay Oprah now and the pH of no idea persona I need you person I now for the negatives are Jamar you know my receptacle and Omo even get to the negative charge or directly open up being h7 the cam people knowing I come people now in the positives are my so in the mind when the Piaget's a change for me and the runner need to recharge of the change Bernie and then me pellets it Damian Elagabalus so illusion a path of my extraction of on the lady they say the molecule element this illusion up and on this will draw if anion exchange chromatography or advantages and disadvantages path on open now and they in came all the advantage one we used to use wastewater treatment number part and the wastewater treatment along the key to that in the chromatographic technique them the useful and ion exchange chromatography so ways to know in the Connecticut charged molecule da and the size in a separate parinamavada and even the purified our own the so on the money went this ways to a way of wastewater treatment plants along the values and then the smaller molecules can be easily separated so the number smaller now on the molecule so I'm gonna be easier respond under charge it and the colon here sorry Paris ironically on a charged idea of a easy on the rampant of Allah he's even Amanda charged of small molecules around the penn state law I know the path or no protein and other biomolecules can be separated and then the molecules which are Jericho I belong the east and on the Roma separate on a protein game in the medieval among the separate from mouth under scuba the designer is Pompano more the moment I set eyes on the hydrophobic molecule seven the nominal separate from another buffer when the kinda path I need a lot of the hydrophobic money comes now at the good a react but nobody I'm gonna be able to be in oil so a separate one of it in the path of majority variation can affect the insulted so minimal narrow path of Asura protein Amin aerosol in the protein long amino acid no salami nos it's all the positive artists elements from the negative article another protein automotive the my heart open even the you know in this in a variation it requires sale another one to positive are causally haven't been area variation recharge Liam do cylinder opacity or aggressive negative area pronounced my don't mistletoe and this idea when they were are this on the charge you variation on this in the result of the phone in the c'mon Bissell minutes so the video dynamics is chromatography petty part-owner c'mon appoints the isoelectric point nine like a problem there mechanism within an activator I've got one day just descend one day chases allow my path at all so first in chromatography petty things you can open on the video or the link description Erica the monochromatic Karina and then went further other type of chromatography him and that has been a terrific play by signature in a molecule coded or a big edge kata Mary positive over negative a hallmark is a solo mesothelioma Norma soon Willa Cather Mary Mary Carol in archery okay so you look at every kid are happier a positive only happy except when the video new position the light commercial as described in the laconic vinegar and think about it you think we should not Instagram to Facebook and the pages or the link description let Kathy follow

Channel: ThiNK Biology ThiNK VISION

Views: 7,146

Keywords: Chromatography, ion exchange chromatography, ThiNK VISION thinkbiology

Id: GZGDL1Yo6ts

Length: 15min 18sec (918 seconds)

Published: Tue Jun 23 2020